Dr Anu Sharma, Dr Sonal Agarwal, Dr K. V. Ingole


Staphylococci are normal inhabitants of human skin and mucous membrane. They play important role as human pathogens causing both
nosocomial as well as community acquired infection. Over the time there has been increased prevalence of MRSA among hospitalized patients
resulting in increased morbidity and mortality among patients.
AIM & OBJECTIVE of the study is to detect the distribution of Staphylococcus in various clinical specimens and to detect antibiotic
susceptibility pattern of these strains against various antibiotics used for the treatment of patients.
MATERIAL & METHOD- Staphylococcus strains were identified using standard procedures and antibiotic susceptibility testing was done on
Mueller Hinton agar by Kirby Bauer disc diffusion method.
RESULT-Total sample received for culture & sensitivity over 6 months from May to October 2018 were 3452 of which 1054 (36.32%) were sterile
and 2198 (63.67%) showed growth. From 2198 (63.67%) samples 350 (15.92%) showed growth of Staphylococcus species. From these
Staphylococcus strain MRSA were 190 (54.28%) followed by MRCONS 86 (24.57%), MSSA 50 (14.28%) and MSCONS 24 (6.85%) Most of the
staphylococcus aureus strains isolated were from pus and wound swab 236 (67.42%) followed by blood 64 (18.28%), urine 16 (4.57%), vaginal
swab 14 (4%), peritoneal fluid 8 (2.28%), sputum 6 (1.71%) and others 6 (1.71%). Male 194 (55.42%) were more commonly infected with
Staphylococcus aureus then females 156 (44.57%).Most common age group affected was between 20 to 30 years accounted to 90 (25.71%) cases
followed by 30 to 40 years 75 (21.42%), 40 to 50 years 60 (17.14%), 10 to 20 years 52 (14.85%), more than 50 years 40 (11.42%) cases, least
number of cases were reported from age group less than 10 years 33 (9.42%). Majority of Staphylococcus aureus strains isolated were from
hospitalized patients of Surgery department 132 (37.71%) followed by Obstetrics and Gynecology 60 (17.14%), OPD 56 (16%), Pediatrics 42
(12%), ENT 20 (5.71%), Medicine 20 (5.71%), TICU 14 (4%), Orthopedics 06 (1.71%). Antibiotic susceptibility pattern of Staphylococcus strains
showed 100% sensitivity to Vancomycin, followed by Gentamicin 81.80%, Ciprofloxacin 50.28%, Erythromycin 35.42%, Clindamycin 34.28%.
Maximum resistance was noted for Cefoxitin 78.85%.


MRSA (Methicillin resistant Staphylococcus aureus), MRCONS (Methicillin resistant coagulase negative Staphylococcus aureus) MSSA (Methicillin sensitive Staphylococcus aureus), MSCONS (Methicillin sensitive coagulase negative Staphylococcus aureus).

Full Text:



Mazhar Salim Al Zoubi, Ibrahim Ali Al Tayyar, Emad Hussein, Alla Al Jabali, Salih Khudairat. Antimicrobial Susceptibility pattern of Staphylococcus aureus isolated from clinical specimens in Northen area of Jorden.Iran J Microbiol. Volume 7, number 5 (October 2015) 265-272.

Brown DF, Edwards DI, Hawkey PM, Morrison D, Ridgway GL, Towner KJ. Guidelines for the laboratory diagnosis and susceptibility testing of methicillin resistant Staphylococcus aureus (MRSA). J Antimicrrob Chemother 2005;56:1000-1018.

Anupurba S, Sen MR, Nath G, Sharma BM, Gulati AK, Mohapatra TM.Prevalence of methicillin resistant Staphylococcus aureus in tertiary referral hospital in eastern Uttar Pradesh. Indian J Med Microbiol 2003;21: 49-51.

Fischetti V.A., Novick R.P., Ferretti J.J., Portnoy D.A. Rood J.I. Gram positive pathogens. Washington, DC: ASM Press: 2000: Pathogenicity factors and their regulation: pp 392-407.

Arbuthnott J.P., Coleman D.C., de Avazedo J.S. Staphylococcus toxins in human disease. Soc Appl Bacteriol Symp Ser. 1990:19:s101-7. [Pubmed].

Lina G, Gillet Y, Vandenesch F, Jones M.E. Floret D, Etienne J . Toxin involvement in Staphylococcal scalded skin syndrome. Clin Infect Dis 1997;25:1369-73.

Novick R.P.. Autoinduction and signal transduction in the regulation of Staphylococcal virulence. Mol Microbiol. 2003;48: 1429-1449 [Pubmed].

Gomes A.R., Vinga S, Zavolan M, de Lencastre H. Analysis of genetic variability of virulence related loci in epidemic clones of methicillin resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2005;49:366-379.[Pubmed].

Hiramatsu K, Chui L, Kuroda M., Ito T. The emergence and evolution of methicillin resistant Staphylococcus aureus. Trends. Microbiol. 2000;9:486-493.

Stefani s, Varaldo P.E. Epidemiology of methicillin resistant Staphylococci in Europe. Clin. Microbiol. Infect. 2003;9:1179-1186.

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disks Susceptibility Tests; Approved Standard. 25th informational supplement CLSI document M100-S25. Wayne, PA: CLSI; 2015.

Xiaoying Xie, Xinlu Dai,Lijia Ni, Baiji Chen, Zhaofan Luo, Yandan Yao, Xiquan Wu, Hongyu Li, Songyin Huang. Molecular epidemiology and Virulence characterstics of Staphylococcus aureus nasal colonization in medical laboratory staff: comparison between Microbiological and non Microbiological laboratories. BMC infectious diseases

Laupland K.B. Gregson DB, ZygunDA, Doig CJ, Mortis G, Church DL. Severe blood stream infections:a population based assessment. Critical care medicine. 2004;32:992-7.

Roder BL, Wandall DA, Frimodt MollerN, EspersenF, Skinhoj P, Rosdahl VT. Clinical featuresof Staphylococcus aureus endocarditis: a 10 year expierence in Denmark. Archivesof Internal Medicine.1999 Mar 8;159:462-469.

Bauer K.A., West JE,Balada Llasat JM, Pancholi P, Stevenson KB, Goff DA. An antimicrobial stewardship program’s impact with rapid polymerase chain reaction methicillin resistant Staphylococcus aureus blood culture test in patients with S. aureus bacteremia. Clinical infectious disease: an official publication of the infectious diseases society of America. 2010; 51: 1074-80.


  • There are currently no refbacks.