COMPARATIVE STUDY OF BRAF MUTATION IN SEROUS TUMORS OF OVARY BY MEANS OF IMMUNOHISTOCHEMISTRY AND REAL TIME POLYMERASE CHAIN REACTION

Sharma Garima, Ahuja Ankur, Deb Prabal, BS Sunita, Trehan Arti, Gahlot Gaurav PS, Joshi Vidushi, Singh Beenu

Abstract


Background:          The purpose of this study was to correlate the evaluation of BRAF V600E by IHC and RT-PCR to correctly categorize, prognosticate and offer the best possible treatment for the serous ovarian tumors thus contributing an attempt for better patient survival and quality of life.

Aims:      To study the expression of BRAF mutation by Immunohistochemistry (IHC) and Real Time Polymerase Chain Reaction (RT-PCR) in various histological grades of serous ovarian tumors

Study design:        This cross section observational study was performed in thirty cases of serous ovarian tumors to access expression of BRAF mutation

Subjects & methods:            To correlate histomorphological grades of serous ovarian tumors with immunohistochemical and RT-PCR results of BRAF mutation in 30 cases.

Statistical Analysis Used: The correlation between BRAF scoring by IHC & RT-PCR, WHO histological grade and subtype of tumor was assessed using Pearson Chi Square Test with statistically significant p-value < 0.05

Results: This study confirmed the higher frequency of BRAF V600E mutations in benign serous tumors, than in high grade tumors as assessed by both IHC and RT-PCR, however no statistically significant correlation could not established between them. It is therefore suggested that the study to be continued with larger number of cases and as a multicentric project with clinical follow-up to comprehend the different facets of BRAF pathway in ovarian neoplasms.

Keywords


Ovarian serous tumors, Low grade serous carcinoma (LGSC), High grade serous carcinoma (HGSC), BRAF V600E, Immunohistochemistry (IHC), Real Time Polymerase Chain Reaction (RT PCR),

Full Text:

PDF

Refbacks

  • There are currently no refbacks.